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Assay Detail

CHEMBL3241348

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of electric eel acetylcholinesterase using acetylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method
27
Total Activities
27
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Electrophorus electricus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL4078)
Type SINGLE PROTEIN
Organism Electrophorus electricus

Publication

Synthesis, cytotoxicity and molecular modelling studies of new phenylcinnamide derivatives as potent inhibitors of cholinesterases.
Saeed A, Mahesar PA, Zaib S, Khan MS, Matin A, Shahid M, Iqbal J.
Eur J Med Chem (2014) 78 : 43 -53
PubMed 24675179 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.38 7.52
Inhibition 9 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502 DONEPEZIL 4.0 1 7.52
CHEMBL3236084 1 6.54
CHEMBL3236088 1 5.85
CHEMBL3236083 1 5.80
CHEMBL3236090 1 5.49
CHEMBL3236098 1 5.43
CHEMBL3236093 1 5.38
CHEMBL3235782 1 5.30
CHEMBL3235785 1 5.22
CHEMBL3236092 1 5.21
CHEMBL1094079 1 5.19
CHEMBL3236099 1 5.17
CHEMBL3235783 1 4.90
CHEMBL3235784 1 4.90
CHEMBL3236087 1 4.89
CHEMBL1094988 1 4.89
CHEMBL278020 NEOSTIGMINE 4.0 1 4.66
CHEMBL3236091 1 4.51
CHEMBL3235786 1 -
CHEMBL3236089 1 -
CHEMBL3236086 1 -
CHEMBL3236085 1 -
CHEMBL3236094 1 -
CHEMBL3236095 1 -
CHEMBL3236096 1 -
CHEMBL3236097 1 -
CHEMBL3236082 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502 DONEPEZIL IC50 = 30.0 nM 7.52
CHEMBL3236084 IC50 = 290.0 nM 6.54
CHEMBL3236088 IC50 = 1410.0 nM 5.85
CHEMBL3236083 IC50 = 1590.0 nM 5.80
CHEMBL3236090 IC50 = 3240.0 nM 5.49
CHEMBL3236098 IC50 = 3720.0 nM 5.43
CHEMBL3236093 IC50 = 4220.0 nM 5.38
CHEMBL3235782 IC50 = 4980.0 nM 5.30
CHEMBL3235785 IC50 = 6020.0 nM 5.22
CHEMBL3236092 IC50 = 6220.0 nM 5.21
CHEMBL1094079 IC50 = 6520.0 nM 5.19
CHEMBL3236099 IC50 = 6690.0 nM 5.17
CHEMBL3235783 IC50 = 12540.0 nM 4.90
CHEMBL3235784 IC50 = 12610.0 nM 4.90
CHEMBL3236087 IC50 = 12770.0 nM 4.89
CHEMBL1094988 IC50 = 12910.0 nM 4.89
CHEMBL278020 NEOSTIGMINE IC50 = 22000.0 nM 4.66
CHEMBL3236091 IC50 = 31200.0 nM 4.51
CHEMBL3235786 Inhibition = 33.6 % -
CHEMBL3236089 Inhibition = 36.4 % -
CHEMBL3236086 Inhibition = 34.2 % -
CHEMBL3236085 Inhibition = 36.5 % -
CHEMBL3236094 Inhibition = 30.3 % -
CHEMBL3236095 Inhibition = 31.2 % -
CHEMBL3236096 Inhibition = 36.6 % -
CHEMBL3236097 Inhibition = 34.1 % -
CHEMBL3236082 Inhibition = 29.2 % -