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Assay Detail

CHEMBL3265996

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at mineralocorticoid receptor (unknown origin) by Gal4-based cellular assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mineralocorticoid receptor (CHEMBL1994)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of (R)-6-(1-(4-cyano-3-methylphenyl)-5-cyclopentyl-4,5-dihydro-1H-pyrazol-3-yl)-2-methoxynicotinic acid, a highly potent and selective nonsteroidal mineralocorticoid receptor antagonist.
Casimiro-Garcia A, Piotrowski DW, Ambler C, Arhancet GB, Banker ME, Banks T, Boustany-Kari CM, Cai C, Chen X, Eudy R, Hepworth D, Hulford CA, Jennings SM, Loria PM, Meyers MJ, Petersen DN, Raheja NK, Sammons M, She L, Song K, Vrieze D, Wei L.
J Med Chem (2014) 57 : 4273 -4288
PubMed 24738581 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.91 8.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3263768 1 8.66
CHEMBL3263760 1 8.57
CHEMBL3263766 1 8.55
CHEMBL3263764 1 8.48
CHEMBL3263762 1 8.43
CHEMBL3263767 1 8.39
CHEMBL3263763 1 8.37
CHEMBL3263757 1 8.36
CHEMBL3263752 1 8.35
CHEMBL3263754 1 8.12
CHEMBL3263750 1 8.05
CHEMBL1215331 PF-03882845 1.0 1 8.04
CHEMBL3263765 1 8.03
CHEMBL3263751 1 7.97
CHEMBL1215130 1 7.82
CHEMBL3263755 1 7.75
CHEMBL3263761 1 7.75
CHEMBL3263756 1 7.67
CHEMBL1215197 1 7.59
CHEMBL3263759 1 7.51
CHEMBL3263758 1 7.25
CHEMBL1095097 EPLERENONE 4.0 1 6.91
CHEMBL3263753 1 5.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3263768 IC50 = 2.2 nM 8.66
CHEMBL3263760 IC50 = 2.7 nM 8.57
CHEMBL3263766 IC50 = 2.8 nM 8.55
CHEMBL3263764 IC50 = 3.3 nM 8.48
CHEMBL3263762 IC50 = 3.7 nM 8.43
CHEMBL3263767 IC50 = 4.1 nM 8.39
CHEMBL3263763 IC50 = 4.3 nM 8.37
CHEMBL3263757 IC50 = 4.4 nM 8.36
CHEMBL3263752 IC50 = 4.5 nM 8.35
CHEMBL3263754 IC50 = 7.6 nM 8.12
CHEMBL3263750 IC50 = 9.0 nM 8.05
CHEMBL1215331 PF-03882845 IC50 = 9.2 nM 8.04
CHEMBL3263765 IC50 = 9.4 nM 8.03
CHEMBL3263751 IC50 = 10.7 nM 7.97
CHEMBL1215130 IC50 = 15.2 nM 7.82
CHEMBL3263755 IC50 = 18.0 nM 7.75
CHEMBL3263761 IC50 = 17.8 nM 7.75
CHEMBL3263756 IC50 = 21.4 nM 7.67
CHEMBL1215197 IC50 = 25.6 nM 7.59
CHEMBL3263759 IC50 = 30.7 nM 7.51
CHEMBL3263758 IC50 = 55.9 nM 7.25
CHEMBL1095097 EPLERENONE IC50 = 122.0 nM 6.91
CHEMBL3263753 IC50 = 4450.0 nM 5.35