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Assay Detail

CHEMBL3267156

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of hexa-His-tagged PAI-1 N152H/K156T/Q321L/M356I mutant (unknown origin) expressed in Escherichia coli BL21(DE3) after 10 mins by uPA-based assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design, synthesis, and SAR of embelin analogues as the inhibitors of PAI-1 (plasminogen activator inhibitor-1).
Chen F, Zhang G, Hong Z, Lin Z, Lei M, Huang M, Hu L.
Bioorg Med Chem Lett (2014) 24 : 2379 -2382
PubMed 24731276 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.77 6.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3263326 1 6.75
CHEMBL3263324 1 6.42
CHEMBL3263334 1 6.29
CHEMBL3263333 1 6.24
CHEMBL3263327 1 6.20
CHEMBL3263325 1 6.12
CHEMBL3263329 1 6.11
CHEMBL3263335 1 6.05
CHEMBL3263332 1 6.04
CHEMBL3263328 1 6.02
CHEMBL2296057 1 5.75
CHEMBL221598 1 5.73
CHEMBL2296056 1 5.41
CHEMBL3263323 1 5.38
CHEMBL2296055 1 5.34
CHEMBL221137 EMBELIN 1 5.31
CHEMBL3263330 1 5.25
CHEMBL3263331 1 5.15
CHEMBL471270 5-O-METHYLEMBELIN 1 4.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3263326 IC50 = 180.0 nM 6.75
CHEMBL3263324 IC50 = 380.0 nM 6.42
CHEMBL3263334 IC50 = 510.0 nM 6.29
CHEMBL3263333 IC50 = 580.0 nM 6.24
CHEMBL3263327 IC50 = 630.0 nM 6.20
CHEMBL3263325 IC50 = 750.0 nM 6.12
CHEMBL3263329 IC50 = 770.0 nM 6.11
CHEMBL3263335 IC50 = 890.0 nM 6.05
CHEMBL3263332 IC50 = 920.0 nM 6.04
CHEMBL3263328 IC50 = 950.0 nM 6.02
CHEMBL2296057 IC50 = 1780.0 nM 5.75
CHEMBL221598 IC50 = 1870.0 nM 5.73
CHEMBL2296056 IC50 = 3870.0 nM 5.41
CHEMBL3263323 IC50 = 4180.0 nM 5.38
CHEMBL2296055 IC50 = 4560.0 nM 5.34
CHEMBL221137 EMBELIN IC50 = 4940.0 nM 5.31
CHEMBL3263330 IC50 = 5680.0 nM 5.25
CHEMBL3263331 IC50 = 7110.0 nM 5.15
CHEMBL471270 5-O-METHYLEMBELIN IC50 = 90190.0 nM 4.04