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Assay Detail

CHEMBL3267626

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Enterococcus faecium VanX expressed in Escherichia coli BL21 (DE3) cells using D-alanyl-alpha-R-phenylthioglycine as substrate by colorimetric assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Enterococcus faecium
Cell Type BL21-DE3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D-alanyl-D-alanine dipeptidase (CHEMBL1681622)
Type SINGLE PROTEIN
Organism Enterococcus faecium

Publication

Cell permeable vanX inhibitors as vancomycin re-sensitizing agents.
Muthyala R, Rastogi N, Shin WS, Peterson ML, Sham YY.
Bioorg Med Chem Lett (2014) 24 : 2535 -2538
PubMed 24751446 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 5.46 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3260854 1 5.82
CHEMBL3260858 1 5.56
CHEMBL3260855 1 5.54
CHEMBL3260856 1 5.21
CHEMBL3260857 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3260854 Ki = 1500.0 nM 5.82
CHEMBL3260858 Ki = 2740.0 nM 5.56
CHEMBL3260855 Ki = 2900.0 nM 5.54
CHEMBL3260856 Ki = 6100.0 nM 5.21
CHEMBL3260857 Ki = 6840.0 nM 5.17