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Assay Detail

CHEMBL3269336

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Fc domain of IgG1-fused human BACE1 (1 to 460) expressed in HEK293 cells preincubated for 10 mins followed by enzyme addition measured after 16 to 24 hrs by FRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 1 (CHEMBL4822)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The evolution of amidine-based brain penetrant BACE1 inhibitors.
Oehlrich D, Prokopcova H, Gijsen HJ.
Bioorg Med Chem Lett (2014) 24 : 2033 -2045
PubMed 24704031 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.69 7.69

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2396989 LY-2886721 1.0 1 7.69

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2396989 LY-2886721 IC50 = 20.3 nM 7.69