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Assay Detail

CHEMBL3269910

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NF-kappaB transactivation in TNF-alpha-stimulated human Jurkat cells preincubated for 2 hrs followed by TNF-alpha challenge measured after 6 hrs by dual luciferase reporter gene assay
2
Total Activities
1
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Jurkat
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Nuclear factor NF-kappa-B complex (CHEMBL2094258)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

Bis(4-hydroxy-2H-chromen-2-one): synthesis and effects on leukemic cell lines proliferation and NF-κB regulation.
Talhi O, Schnekenburger M, Panning J, Pinto DG, Fernandes JA, Almeida Paz FA, Jacob C, Diederich M, Silva AM.
Bioorg Med Chem (2014) 22 : 3008 -3015
PubMed 24775915 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.72 4.72
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3264621 2 4.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3264621 IC50 = 19000.0 nM 4.72
CHEMBL3264621 Inhibition - % -