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Assay Detail

CHEMBL3270692

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]N-methylspiperone from human dopamine D2 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(2) dopamine receptor (CHEMBL217)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tranylcypromine substituted cis-hydroxycyclobutylnaphthamides as potent and selective dopamine D₃ receptor antagonists.
Chen J, Levant B, Jiang C, Keck TM, Newman AH, Wang S.
J Med Chem (2014) 57 : 4962 -4968
PubMed 24848155 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 7.29 10.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8946 ETICLOPRIDE 2.0 1 10.07
CHEMBL267932 MESPIPERONE 2.0 1 9.88
CHEMBL1255588 1 8.59
CHEMBL8809 RACLOPRIDE 2.0 1 7.90
CHEMBL3265067 1 6.21
CHEMBL3265063 1 6.18
CHEMBL1627305 1 6.13
CHEMBL3265064 1 6.04
CHEMBL242644 1 5.96
CHEMBL3265065 1 5.91

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8946 ETICLOPRIDE Ki = 0.086 nM 10.07
CHEMBL267932 MESPIPERONE Ki = 0.133 nM 9.88
CHEMBL1255588 Ki = 2.58 nM 8.59
CHEMBL8809 RACLOPRIDE Ki = 12.7 nM 7.90
CHEMBL3265067 Ki = 623.0 nM 6.21
CHEMBL3265063 Ki = 667.0 nM 6.18
CHEMBL1627305 Ki = 746.0 nM 6.13
CHEMBL3265064 Ki = 923.0 nM 6.04
CHEMBL242644 Ki = 1090.0 nM 5.96
CHEMBL3265065 Ki = 1220.0 nM 5.91