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Assay Detail

CHEMBL3279769

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of Escherichia coli thymidylate synthetase using 5-[3H]-dUMP as substrate after 7 mins by scintillation counting analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthetic inhibitors of Escherichia coli, calf thymus, and Ehrlich ascites tumor thymidylate synthetase.
Kampf A, Barfknecht RL, Shaffer PJ, Osaki S, Mertes MP.
J Med Chem (1976) 19 : 903 -908
PubMed 781246 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 4.99 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1236552 1 7.00
CHEMBL1160818 1 5.40
CHEMBL3273353 1 4.75
CHEMBL3272995 1 4.60
CHEMBL3273352 1 4.60
CHEMBL3272994 1 4.38
CHEMBL3272996 1 4.19
CHEMBL3273351 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1236552 Ki = 100.0 nM 7.00
CHEMBL1160818 Ki = 4000.0 nM 5.40
CHEMBL3273353 Ki = 18000.0 nM 4.75
CHEMBL3272995 Ki = 25000.0 nM 4.60
CHEMBL3273352 Ki = 25000.0 nM 4.60
CHEMBL3272994 Ki = 42000.0 nM 4.38
CHEMBL3272996 Ki = 64000.0 nM 4.19
CHEMBL3273351 Ki = 135000.0 nM -