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Assay Detail

CHEMBL3292468

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SGLT2 expressed in CHOK1 cells assessed as inhibition of [14C]-AMG uptake after 3 hrs by microbeta scintillation counting analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium/glucose cotransporter 2 (CHEMBL3884)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The design and synthesis of novel SGLT2 inhibitors: C-glycosides with benzyltriazolopyridinone and phenylhydantoin as the aglycone moieties.
Guo C, Hu M, DeOrazio RJ, Usyatinsky A, Fitzpatrick K, Zhang Z, Maeng JH, Kitchen DB, Tom S, Luche M, Khmelnitsky Y, Mhyre AJ, Guzzo PR, Liu S.
Bioorg Med Chem (2014) 22 : 3414 -3422
PubMed 24842618 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.63 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL429910 DAPAGLIFLOZIN 4.0 1 9.00
CHEMBL3288755 1 7.96
CHEMBL3288756 1 7.63
CHEMBL3288752 1 7.48
CHEMBL3288738 1 7.10
CHEMBL3288737 1 7.08
CHEMBL3288744 1 7.05
CHEMBL3288739 1 6.97
CHEMBL3288745 1 6.96
CHEMBL3288740 1 6.92
CHEMBL3288741 1 6.85
CHEMBL3288742 1 6.65
CHEMBL3288754 1 6.61
CHEMBL3288746 1 6.60
CHEMBL3288753 1 6.48
CHEMBL3288757 1 6.31
CHEMBL3288743 1 6.12
CHEMBL3288751 1 5.48
CHEMBL3288747 1 5.26
CHEMBL3288748 1 5.24
CHEMBL3288750 1 5.05
CHEMBL3288749 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL429910 DAPAGLIFLOZIN IC50 = 1.0 nM 9.00
CHEMBL3288755 IC50 = 10.9 nM 7.96
CHEMBL3288756 IC50 = 23.6 nM 7.63
CHEMBL3288752 IC50 = 33.0 nM 7.48
CHEMBL3288738 IC50 = 80.0 nM 7.10
CHEMBL3288737 IC50 = 83.0 nM 7.08
CHEMBL3288744 IC50 = 89.0 nM 7.05
CHEMBL3288739 IC50 = 107.0 nM 6.97
CHEMBL3288745 IC50 = 110.0 nM 6.96
CHEMBL3288740 IC50 = 120.0 nM 6.92
CHEMBL3288741 IC50 = 141.0 nM 6.85
CHEMBL3288742 IC50 = 222.0 nM 6.65
CHEMBL3288754 IC50 = 248.0 nM 6.61
CHEMBL3288746 IC50 = 252.0 nM 6.60
CHEMBL3288753 IC50 = 328.0 nM 6.48
CHEMBL3288757 IC50 = 489.0 nM 6.31
CHEMBL3288743 IC50 = 762.0 nM 6.12
CHEMBL3288751 IC50 = 3296.0 nM 5.48
CHEMBL3288747 IC50 = 5478.0 nM 5.26
CHEMBL3288748 IC50 = 5699.0 nM 5.24
CHEMBL3288750 IC50 = 9000.0 nM 5.05
CHEMBL3288749 IC50 = 9916.0 nM 5.00