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Assay Detail

CHEMBL3295172

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of microsomal PGES1 isolated from IL-1beta-stimulated human A549 cells preincubated for 15 mins followed by substrate addition measured after 1 min by RP-HPLC analysis
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E synthase (CHEMBL5658)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tetra- and pentacyclic triterpene acids from the ancient anti-inflammatory remedy frankincense as inhibitors of microsomal prostaglandin E(2) synthase-1.
Verhoff M, Seitz S, Paul M, Noha SM, Jauch J, Schuster D, Werz O.
J Nat Prod (2014) 77 : 1445 -1451
PubMed 24844534 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.72 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3289100 1 6.40
CHEMBL3289097 1 6.40
CHEMBL566929 BETA-ELEMONIC ACID 1 6.05
CHEMBL3289107 1 6.05
CHEMBL3289103 1 6.00
CHEMBL3289096 1 5.96
CHEMBL3289098 1 5.92
CHEMBL29097 1 5.62
CHEMBL236906 1 5.52
CHEMBL3289099 1 5.52
CHEMBL437964 11-KETO BOSWELLIC ACID 1 5.52
CHEMBL237111 AKBA -1.0 1 5.52
CHEMBL267225 BOSWELLIC ACID 2.0 1 5.30
CHEMBL3289105 1 5.07
CHEMBL3289106 1 5.00
CHEMBL3289104 1 -
CHEMBL3289102 1 -
CHEMBL3289101 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3289100 IC50 = 400.0 nM 6.40
CHEMBL3289097 IC50 = 400.0 nM 6.40
CHEMBL566929 BETA-ELEMONIC ACID IC50 = 900.0 nM 6.05
CHEMBL3289107 IC50 = 900.0 nM 6.05
CHEMBL3289103 IC50 = 1000.0 nM 6.00
CHEMBL3289096 IC50 = 1100.0 nM 5.96
CHEMBL3289098 IC50 = 1200.0 nM 5.92
CHEMBL29097 IC50 = 2400.0 nM 5.62
CHEMBL236906 IC50 = 3000.0 nM 5.52
CHEMBL3289099 IC50 = 3000.0 nM 5.52
CHEMBL437964 11-KETO BOSWELLIC ACID IC50 = 3000.0 nM 5.52
CHEMBL237111 AKBA IC50 = 3000.0 nM 5.52
CHEMBL267225 BOSWELLIC ACID IC50 = 5000.0 nM 5.30
CHEMBL3289105 IC50 = 8500.0 nM 5.07
CHEMBL3289106 IC50 = 10000.0 nM 5.00
CHEMBL3289104 IC50 > 10000.0 nM -
CHEMBL3289102 IC50 > 10000.0 nM -
CHEMBL3289101 IC50 > 10000.0 nM -