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Assay Detail

CHEMBL3299604

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]BE2254 from human alpha1a receptor expressed in HEK293 cells
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alpha-1A adrenergic receptor (CHEMBL229)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

High affinity ligands and potent antagonists for the α1D-adrenergic receptor. Novel 3,8-disubstituted [1]benzothieno[3,2-d]pyrimidine derivatives.
Romeo G, Salerno L, Pittalà V, Modica MN, Siracusa MA, Materia L, Buccioni M, Marucci G, Minneman KP.
Eur J Med Chem (2014) 83 : 419 -432
PubMed 24992070 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 8.71 9.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3298750 1 9.49
CHEMBL3298023 1 9.04
CHEMBL3297829 1 9.03
CHEMBL3298024 1 9.00
CHEMBL3298745 1 8.96
CHEMBL3298749 1 8.96
CHEMBL3298746 1 8.83
CHEMBL220221 1 8.83
CHEMBL3298017 1 8.79
CHEMBL3298019 1 8.77
CHEMBL220781 1 8.48
CHEMBL3298021 1 8.47
CHEMBL3298022 1 8.45
CHEMBL3298020 1 8.41
CHEMBL3298748 1 8.28
CHEMBL3298018 1 8.16
CHEMBL3298747 1 8.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3298750 Ki = 0.3236 nM 9.49
CHEMBL3298023 Ki = 0.912 nM 9.04
CHEMBL3297829 Ki = 0.9333 nM 9.03
CHEMBL3298024 Ki = 1.0 nM 9.00
CHEMBL3298745 Ki = 1.096 nM 8.96
CHEMBL3298749 Ki = 1.096 nM 8.96
CHEMBL3298746 Ki = 1.479 nM 8.83
CHEMBL220221 Ki = 1.479 nM 8.83
CHEMBL3298017 Ki = 1.622 nM 8.79
CHEMBL3298019 Ki = 1.698 nM 8.77
CHEMBL220781 Ki = 3.311 nM 8.48
CHEMBL3298021 Ki = 3.388 nM 8.47
CHEMBL3298022 Ki = 3.548 nM 8.45
CHEMBL3298020 Ki = 3.89 nM 8.41
CHEMBL3298748 Ki = 5.248 nM 8.28
CHEMBL3298018 Ki = 6.918 nM 8.16
CHEMBL3298747 Ki = 7.079 nM 8.15