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Assay Detail

CHEMBL3300336

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antimalarial activity against Plasmodium falciparum NFS4 assessed as inhibition of [3H]hypoxanthine incorporation preincubated for 48 hrs measured 24 hrs post [3H]hypoxanthine addition by liquid scintillation counting
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Plasmodium falciparum
Confidence 1 — Organism
Curated By Autocuration

Target

Plasmodium falciparum (CHEMBL364)
Type ORGANISM
Organism Plasmodium falciparum

Publication

Novel synthetic route for antimalarial benzo[a]phenoxazine derivative SSJ-183 and two active metabolites.
Mizukawa Y, Ge JF, Bakar Md A, Itoh I, Scheurer C, Wittlin S, Brun R, Matsuoka H, Ihara M.
Bioorg Med Chem (2014) 22 : 3749 -3752
PubMed 24856305 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 8.01 8.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL191083 METHYLENE BLUE CATION 4.0 1 8.38
CHEMBL3298603 1 8.12
CHEMBL1780224 1 7.96
CHEMBL76 CHLOROQUINE 4.0 1 7.96
CHEMBL3298604 1 7.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL191083 METHYLENE BLUE CATION IC50 = 4.2 nM 8.38
CHEMBL3298603 IC50 = 7.5 nM 8.12
CHEMBL1780224 IC50 = 11.0 nM 7.96
CHEMBL76 CHLOROQUINE IC50 = 11.0 nM 7.96
CHEMBL3298604 IC50 = 24.0 nM 7.62