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Assay Detail

CHEMBL3301449

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
MMV: Primary screening was performed with each compound at a 30 uM final concentration and in triplicate in sealed 96-well polystyrene microtiter plates. Parasite inocula (100 ul) comprising 20000 parasites/ml were added to each well and grown at 37 degrees C in a humidified atmosphere of 5% CO2 for 72 h. Positive controls consisted of metronidazole at 1 mg/ml. The growth of E. histolytica trophozoites in each well was determined microscopically by measuring the diameters of the confluent cells in drug-containing wells relative to those in the nega- tive-control wells.
400
Total Activities
400
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Entamoeba histolytica
Confidence 1 — Organism
Curated By Autocuration

Target

Entamoeba histolytica (CHEMBL612853)
Type ORGANISM
Organism Entamoeba histolytica

Publication

MMV Malaria Box screening against Toxoplasma gondii and Entamoeba histolytica.
Boyom, F.F., Fokou, P.V.T., Tchokouaha, L.R.Y., Spangenberg, T., Mfopa, A.N., Kouipou, R.M.T., Mbouna, C.J., Donkeng Donfack, V.F., Zollo, P.H.A. University of Yaoundé, IMPM Yaoundé and MMV.

· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 400 4.89 4.97

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL579443 1 4.97
CHEMBL582666 1 4.81
CHEMBL124006 1 -
CHEMBL600305 1 -
CHEMBL526981 1 -
CHEMBL532879 1 -
CHEMBL591637 1 -
CHEMBL588855 1 -
CHEMBL530973 1 -
CHEMBL494669 1 -
CHEMBL546168 1 -
CHEMBL601957 1 -
CHEMBL533563 1 -
CHEMBL547488 1 -
CHEMBL2028051 1 -
CHEMBL581175 1 -
CHEMBL590674 1 -
CHEMBL587410 1 -
CHEMBL529732 1 -
CHEMBL585264 1 -
CHEMBL580580 1 -
CHEMBL587989 1 -
CHEMBL529773 1 -
CHEMBL535730 1 -
CHEMBL2028042 1 -
CHEMBL604323 1 -
CHEMBL535514 1 -
CHEMBL2028053 1 -
CHEMBL592105 METHYLROSANILINE -1.0 1 -
CHEMBL587288 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL579443 IC50 = 10660.0 nM 4.97
CHEMBL582666 IC50 = 15580.0 nM 4.81
CHEMBL582119 IC50 > 30000.0 nM -
CHEMBL338094 IC50 > 30000.0 nM -
CHEMBL592123 IC50 > 30000.0 nM -
CHEMBL585983 IC50 > 30000.0 nM -
CHEMBL587485 IC50 > 30000.0 nM -
CHEMBL601806 IC50 > 30000.0 nM -
CHEMBL601743 IC50 > 30000.0 nM -
CHEMBL580516 IC50 > 30000.0 nM -
CHEMBL582180 IC50 > 30000.0 nM -
CHEMBL534319 IC50 > 30000.0 nM -
CHEMBL548209 IC50 > 30000.0 nM -
CHEMBL600488 IC50 > 30000.0 nM -
CHEMBL585622 IC50 > 30000.0 nM -
CHEMBL548374 IC50 > 30000.0 nM -
CHEMBL590683 IC50 > 30000.0 nM -
CHEMBL600549 IC50 > 30000.0 nM -
CHEMBL589422 IC50 > 30000.0 nM -
CHEMBL582495 IC50 > 30000.0 nM -
CHEMBL579300 IC50 > 30000.0 nM -
CHEMBL586178 IC50 > 30000.0 nM -
CHEMBL583555 IC50 > 30000.0 nM -
CHEMBL525692 IC50 > 30000.0 nM -
CHEMBL603686 IC50 > 30000.0 nM -
CHEMBL584655 IC50 > 30000.0 nM -
CHEMBL579760 IC50 > 30000.0 nM -
CHEMBL591623 IC50 > 30000.0 nM -
CHEMBL527541 IC50 > 30000.0 nM -
CHEMBL601325 IC50 > 30000.0 nM -
CHEMBL586078 IC50 > 30000.0 nM -
CHEMBL546531 IC50 > 30000.0 nM -
CHEMBL598881 IC50 > 30000.0 nM -
CHEMBL592550 IC50 > 30000.0 nM -
CHEMBL532015 IC50 > 30000.0 nM -
CHEMBL602234 IC50 > 30000.0 nM -
CHEMBL591887 IC50 > 30000.0 nM -
CHEMBL607688 IC50 > 30000.0 nM -
CHEMBL589723 IC50 > 30000.0 nM -
CHEMBL526800 IC50 > 30000.0 nM -
CHEMBL601492 IC50 > 30000.0 nM -
CHEMBL547266 IC50 > 30000.0 nM -
CHEMBL592808 IC50 > 30000.0 nM -
CHEMBL599886 IC50 > 30000.0 nM -
CHEMBL584235 IC50 > 30000.0 nM -
CHEMBL528437 IC50 > 30000.0 nM -
CHEMBL600174 IC50 > 30000.0 nM -
CHEMBL601156 IC50 > 30000.0 nM -
CHEMBL600356 IC50 > 30000.0 nM -
CHEMBL585839 IC50 > 30000.0 nM -