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Assay Detail

CHEMBL3301457

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Functional
MMV: P. falciparum NF54 functionally mature Stage V gametocytes were incubated for 24 hr in the presence of the test compounds. Gamete formation was then triggered by addition of 2uM xantheurenic acid and temperature decrease to ~20C. Female gamete formation was recorded 24 hr later by automated microscopy detection of surface labelling of female gametes with a Cy3-conjugated antibody reactive to Pfs25. All compounds were tested in 4 independent experiments and data presented here is the mean. IC50s determined for selected compounds.
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Plasmodium falciparum
Confidence 1 — Organism
Curated By Autocuration

Target

Plasmodium falciparum (CHEMBL364)
Type ORGANISM
Organism Plasmodium falciparum

Publication

MMV Malaria Box gametocyte functional viability assay screening.
Imperial-Medicines for Malaria Venture Centre of Excellence, Imperial College, London.

· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 6.25 6.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL582767 1 6.80
CHEMBL602580 1 6.72
CHEMBL530223 1 6.42
CHEMBL592105 METHYLROSANILINE -1.0 1 6.35
CHEMBL1437888 1 6.33
CHEMBL601325 1 6.21
CHEMBL124006 1 5.97
CHEMBL1545915 1 5.19
CHEMBL524973 1 -
CHEMBL586031 1 -
CHEMBL601378 1 -
CHEMBL597855 1 -
CHEMBL206540 1 -
CHEMBL581175 1 -
CHEMBL586000 1 -
CHEMBL586310 1 -
CHEMBL530973 1 -
CHEMBL125044 1 -
CHEMBL549210 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL582767 IC50 = 159.4 nM 6.80
CHEMBL602580 IC50 = 188.8 nM 6.72
CHEMBL530223 IC50 = 383.9 nM 6.42
CHEMBL592105 METHYLROSANILINE IC50 = 445.5 nM 6.35
CHEMBL1437888 IC50 = 473.1 nM 6.33
CHEMBL601325 IC50 = 614.1 nM 6.21
CHEMBL124006 IC50 = 1074.0 nM 5.97
CHEMBL1545915 IC50 = 6452.0 nM 5.19
CHEMBL524973 IC50 > 50000.0 nM -
CHEMBL586031 IC50 > 50000.0 nM -
CHEMBL601378 IC50 > 50000.0 nM -
CHEMBL597855 IC50 > 50000.0 nM -
CHEMBL206540 IC50 > 50000.0 nM -
CHEMBL581175 IC50 > 50000.0 nM -
CHEMBL586000 IC50 > 50000.0 nM -
CHEMBL586310 IC50 > 50000.0 nM -
CHEMBL530973 IC50 > 50000.0 nM -
CHEMBL125044 IC50 > 50000.0 nM -
CHEMBL549210 IC50 > 50000.0 nM -