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Assay Detail

CHEMBL3362602

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant MraY overexpressed in Escherichia coli membranes by continuous fluorescence assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Phospho-N-acetylmuramoyl-pentapeptide-transferase (CHEMBL3559642)
Type SINGLE PROTEIN
Organism Escherichia coli

Publication

Identification of novel inhibitors of phospho-MurNAc-pentapeptide translocase MraY from library screening: Isoquinoline alkaloid michellamine B and xanthene dye phloxine B.
Mihalyi A, Jamshidi S, Slikas J, Bugg TD.
Bioorg Med Chem (2014) 22 : 4566 -4571
PubMed 25127465 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 4.50 4.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1205178 1 4.50
CHEMBL295292 MICHELLAMINE A 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1205178 IC50 = 32000.0 nM 4.50
CHEMBL295292 MICHELLAMINE A IC50 = 456000.0 nM -