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Assay Detail

CHEMBL3363899

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of norepinephrine reuptake at human NET expressed in HEK293 cells after 15 mins by fluorescence neurotransmitter transporter assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent noradrenaline transporter (CHEMBL222)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of 3-phenethylazetidine derivatives as triple reuptake inhibitors.
Yun J, Han M, Song C, Cheon SH, Choi K, Hahn HG.
Bioorg Med Chem Lett (2014) 24 : 3234 -3237
PubMed 24974340 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.12 7.97

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3310482 1 7.97
CHEMBL3310485 1 7.84
CHEMBL3310489 1 7.83
CHEMBL3310484 1 7.80
CHEMBL295467 NISOXETINE 2.0 1 7.70
CHEMBL3310483 1 7.52
CHEMBL3310487 1 7.51
CHEMBL3310486 1 7.49
CHEMBL3310490 1 7.48
CHEMBL3309701 1 7.47
CHEMBL3310478 1 7.47
CHEMBL3309702 1 7.43
CHEMBL3310488 1 7.16
CHEMBL592374 AMITIFADINE 2.0 1 6.84
CHEMBL1175 DULOXETINE 4.0 1 6.29
CHEMBL543876 1 5.84
CHEMBL41 FLUOXETINE 4.0 1 5.36
CHEMBL637 VENLAFAXINE 4.0 1 5.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3310482 IC50 = 10.6 nM 7.97
CHEMBL3310485 IC50 = 14.6 nM 7.84
CHEMBL3310489 IC50 = 14.7 nM 7.83
CHEMBL3310484 IC50 = 15.9 nM 7.80
CHEMBL295467 NISOXETINE IC50 = 20.0 nM 7.70
CHEMBL3310483 IC50 = 30.0 nM 7.52
CHEMBL3310487 IC50 = 30.8 nM 7.51
CHEMBL3310486 IC50 = 32.5 nM 7.49
CHEMBL3310490 IC50 = 33.1 nM 7.48
CHEMBL3309701 IC50 = 33.5 nM 7.47
CHEMBL3310478 IC50 = 33.7 nM 7.47
CHEMBL3309702 IC50 = 37.2 nM 7.43
CHEMBL3310488 IC50 = 68.6 nM 7.16
CHEMBL592374 AMITIFADINE IC50 = 143.0 nM 6.84
CHEMBL1175 DULOXETINE IC50 = 515.0 nM 6.29
CHEMBL543876 IC50 = 1460.0 nM 5.84
CHEMBL41 FLUOXETINE IC50 = 4410.0 nM 5.36
CHEMBL637 VENLAFAXINE IC50 = 5722.0 nM 5.24