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Assay Detail

CHEMBL3365756

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant NEP using Suc-Ala-Ala-Phe-AMC as substrate after 30 mins by fluorimetry
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neprilysin (CHEMBL1944)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

New orally active dual enkephalinase inhibitors (DENKIs) for central and peripheral pain treatment.
Poras H, Bonnard E, Dangé E, Fournié-Zaluski MC, Roques BP.
J Med Chem (2014) 57 : 5748 -5763
PubMed 24927250 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 8.26 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL51715 1 9.15
CHEMBL298827 (S)-THIORPHAN 1 8.82
CHEMBL3298934 1 8.82
CHEMBL3298935 1 8.70
CHEMBL3298860 1 8.60
CHEMBL3298859 1 8.54
CHEMBL3298940 1 8.46
CHEMBL3298937 1 8.09
CHEMBL3298941 1 7.75
CHEMBL3298936 1 7.62
CHEMBL3298938 1 7.36
CHEMBL3298939 1 7.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL51715 Ki = 0.7 nM 9.15
CHEMBL298827 (S)-THIORPHAN Ki = 1.5 nM 8.82
CHEMBL3298934 Ki = 1.5 nM 8.82
CHEMBL3298935 Ki = 2.0 nM 8.70
CHEMBL3298860 Ki = 2.5 nM 8.60
CHEMBL3298859 Ki = 2.9 nM 8.54
CHEMBL3298940 Ki = 3.45 nM 8.46
CHEMBL3298937 Ki = 8.2 nM 8.09
CHEMBL3298941 Ki = 17.9 nM 7.75
CHEMBL3298936 Ki = 24.0 nM 7.62
CHEMBL3298938 Ki = 44.1 nM 7.36
CHEMBL3298939 Ki = 59.0 nM 7.23