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Assay Detail

CHEMBL3366023

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 5-LOX in human PMNL cells assessed as reduction in 5-LOX product formation pre-incubated for 15 mins by HPLC based cell-free assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type PMNL
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL215)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of pirinixic acid derivatives bearing a 2-aminothiazole moiety combines dual PPARα/γ activation and dual 5-LO/mPGES-1 inhibition.
Hanke T, Lamers C, Gomez RC, Schneider G, Werz O, Schubert-Zsilavecz M.
Bioorg Med Chem Lett (2014) 24 : 3757 -3763
PubMed 25037914 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.72 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3094412 1 6.52
CHEMBL3094431 1 6.10
CHEMBL3094426 1 5.80
CHEMBL3094417 1 5.75
CHEMBL3094410 1 5.72
CHEMBL3094425 1 5.72
CHEMBL3091498 1 5.70
CHEMBL3094418 1 5.64
CHEMBL3094415 1 5.64
CHEMBL3094416 1 5.60
CHEMBL3091499 1 5.52
CHEMBL3094432 1 5.42
CHEMBL3094433 1 5.18
CHEMBL3318411 1 -
CHEMBL3091496 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3094412 IC50 = 300.0 nM 6.52
CHEMBL3094431 IC50 = 800.0 nM 6.10
CHEMBL3094426 IC50 = 1600.0 nM 5.80
CHEMBL3094417 IC50 = 1800.0 nM 5.75
CHEMBL3094410 IC50 = 1900.0 nM 5.72
CHEMBL3094425 IC50 = 1900.0 nM 5.72
CHEMBL3091498 IC50 = 2000.0 nM 5.70
CHEMBL3094418 IC50 = 2300.0 nM 5.64
CHEMBL3094415 IC50 = 2300.0 nM 5.64
CHEMBL3094416 IC50 = 2500.0 nM 5.60
CHEMBL3091499 IC50 = 3000.0 nM 5.52
CHEMBL3094432 IC50 = 3800.0 nM 5.42
CHEMBL3094433 IC50 = 6600.0 nM 5.18
CHEMBL3318411 IC50 > 10000.0 nM -
CHEMBL3091496 IC50 > 10000.0 nM -