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Assay Detail

CHEMBL3366540

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of human full-length PXR transfected in human HepG2 cells after 24 hrs by luciferase reporter gene assay
17
Total Activities
17
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear receptor subfamily 1 group I member 2 (CHEMBL3401)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of the CCR1 antagonist, BMS-817399, for the treatment of rheumatoid arthritis.
Santella JB, Gardner DS, Duncia JV, Wu H, Dhar M, Cavallaro C, Tebben AJ, Carter PH, Barrish JC, Yarde M, Briceno SW, Cvijic ME, Grafstrom RR, Liu R, Patel SR, Watson AJ, Yang G, Rose AV, Vickery RD, Caceres-Cortes J, Caporuscio C, Camac DM, Khan JA, An Y, Foster WR, Davies P, Hynes J.
J Med Chem (2014) 57 : 7550 -7564
PubMed 25101488 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 5.06 5.64
EC20 5 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2398716 1 5.64
CHEMBL3334727 1 5.24
CHEMBL3334822 1 5.11
CHEMBL3334815 1 4.72
CHEMBL3334825 1 4.60
CHEMBL3334725 1 -
CHEMBL3334726 1 -
CHEMBL3334728 1 -
CHEMBL3334816 1 -
CHEMBL3334817 1 -
CHEMBL3334724 1 -
CHEMBL3334819 1 -
CHEMBL3334820 1 -
CHEMBL3334821 1 -
CHEMBL3334823 1 -
CHEMBL3334824 BMS-817399 2.0 1 -
CHEMBL3334818 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2398716 EC50 = 2300.0 nM 5.64
CHEMBL3334727 EC50 = 5700.0 nM 5.24
CHEMBL3334822 EC50 = 7800.0 nM 5.11
CHEMBL3334815 EC50 = 19000.0 nM 4.72
CHEMBL3334825 EC50 = 25000.0 nM 4.60
CHEMBL3334725 EC20 = 7.1 uM -
CHEMBL3334726 EC50 > 50000.0 nM -
CHEMBL3334728 EC50 > 50000.0 nM -
CHEMBL3334816 EC20 = 5.2 uM -
CHEMBL3334817 EC20 = 8.7 uM -
CHEMBL3334724 EC50 - -
CHEMBL3334819 EC20 = 0.8 uM -
CHEMBL3334820 EC50 - -
CHEMBL3334821 EC50 > 25000.0 nM -
CHEMBL3334823 EC50 > 25000.0 nM -
CHEMBL3334824 BMS-817399 EC50 > 25000.0 nM -
CHEMBL3334818 EC20 = 2.2 uM -