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Assay Detail

CHEMBL3368588

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human PPARgamma LBD expressed in COS7 cells after 12 hrs by Dual-Glo luciferase assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Molecular determinants for improved activity at PPARα: structure-activity relationship of pirinixic acid derivatives, docking study and site-directed mutagenesis of PPARα.
Lamers C, Dittrich M, Steri R, Proschak E, Schubert-Zsilavecz M.
Bioorg Med Chem Lett (2014) 24 : 4048 -4052
PubMed 25022880 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 5.57 6.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3318041 1 6.31
CHEMBL3318038 1 6.16
CHEMBL3318037 1 5.97
CHEMBL3318039 1 5.92
CHEMBL3318040 1 5.63
CHEMBL459972 1 5.52
CHEMBL518038 1 5.44
CHEMBL3318036 1 4.91
CHEMBL295416 PIRINIXIC ACID 2.0 1 4.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3318041 EC50 = 490.0 nM 6.31
CHEMBL3318038 EC50 = 690.0 nM 6.16
CHEMBL3318037 EC50 = 1060.0 nM 5.97
CHEMBL3318039 EC50 = 1200.0 nM 5.92
CHEMBL3318040 EC50 = 2360.0 nM 5.63
CHEMBL459972 EC50 = 3000.0 nM 5.52
CHEMBL518038 EC50 = 3600.0 nM 5.44
CHEMBL3318036 EC50 = 12400.0 nM 4.91
CHEMBL295416 PIRINIXIC ACID EC50 = 53700.0 nM 4.27