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Assay Detail

CHEMBL3369491

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HDAC in human HeLa cells nuclear extracts incubated for 30 mins by fluorescent assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Histone deacetylase (CHEMBL2093865)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Indole-3-ethylsulfamoylphenylacrylamides: potent histone deacetylase inhibitors with anti-inflammatory activity.
Mehndiratta S, Hsieh YL, Liu YM, Wang AW, Lee HY, Liang LY, Kumar S, Teng CM, Yang CR, Liou JP.
Eur J Med Chem (2014) 85 : 468 -479
PubMed 25113875 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.87 8.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3329991 1 8.55
CHEMBL3329990 1 8.48
CHEMBL3329992 1 8.47
CHEMBL3329999 1 8.24
CHEMBL3329998 1 8.18
CHEMBL3329994 1 8.14
CHEMBL3330002 1 8.12
CHEMBL3329993 1 8.10
CHEMBL3329997 1 8.04
CHEMBL408513 BELINOSTAT 4.0 1 7.58
CHEMBL3329996 1 7.53
CHEMBL3330000 1 7.36
CHEMBL3329995 1 7.32
CHEMBL3330001 1 6.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3329991 IC50 = 2.8 nM 8.55
CHEMBL3329990 IC50 = 3.3 nM 8.48
CHEMBL3329992 IC50 = 3.4 nM 8.47
CHEMBL3329999 IC50 = 5.7 nM 8.24
CHEMBL3329998 IC50 = 6.6 nM 8.18
CHEMBL3329994 IC50 = 7.3 nM 8.14
CHEMBL3330002 IC50 = 7.5 nM 8.12
CHEMBL3329993 IC50 = 7.9 nM 8.10
CHEMBL3329997 IC50 = 9.2 nM 8.04
CHEMBL408513 BELINOSTAT IC50 = 26.4 nM 7.58
CHEMBL3329996 IC50 = 29.6 nM 7.53
CHEMBL3330000 IC50 = 43.9 nM 7.36
CHEMBL3329995 IC50 = 47.4 nM 7.32
CHEMBL3330001 IC50 = 771.8 nM 6.11