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Assay Detail

CHEMBL3370212

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of LPS-induced NO production in mouse J774A1 cells compound preincubated for 1 hr before LPS treatment by Griess reaction
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type J774.A1
Confidence 1 — Organism
Curated By Autocuration

Target

J774.A1 (CHEMBL614040)
Type CELL-LINE
Organism Mus musculus

Publication

Oleanolic acid analogs as NO, TNF-α and IL-1β inhibitors: synthesis, biological evaluation and docking studies.
Bhandari P, Patel NK, Gangwal RP, Sangamwar AT, Bhutani KK.
Bioorg Med Chem Lett (2014) 24 : 4114 -4119
PubMed 25113933 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 4.60 4.97

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3322658 1 4.97
CHEMBL3322657 1 4.80
CHEMBL486822 ACETYL OLEANOLIC ACID 1 4.78
CHEMBL3322656 1 4.75
CHEMBL3322659 1 4.74
CHEMBL140 CURCUMIN 3.0 1 4.73
CHEMBL555056 1 4.68
CHEMBL3322655 1 4.60
CHEMBL3322661 1 4.58
CHEMBL3322662 1 4.56
CHEMBL3322660 1 4.53
CHEMBL1631518 1 4.47
CHEMBL168 OLEANOLIC_ACID 1 4.45
CHEMBL470029 OLEANONIC ACID 1 4.41
CHEMBL1797142 1 4.38
CHEMBL7890 L-NAME 2.0 1 4.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3322658 IC50 = 10800.0 nM 4.97
CHEMBL3322657 IC50 = 15900.0 nM 4.80
CHEMBL486822 ACETYL OLEANOLIC ACID IC50 = 16800.0 nM 4.78
CHEMBL3322656 IC50 = 17700.0 nM 4.75
CHEMBL3322659 IC50 = 18400.0 nM 4.74
CHEMBL140 CURCUMIN IC50 = 18520.0 nM 4.73
CHEMBL555056 IC50 = 21000.0 nM 4.68
CHEMBL3322655 IC50 = 24900.0 nM 4.60
CHEMBL3322661 IC50 = 26100.0 nM 4.58
CHEMBL3322662 IC50 = 27400.0 nM 4.56
CHEMBL3322660 IC50 = 29400.0 nM 4.53
CHEMBL1631518 IC50 = 33500.0 nM 4.47
CHEMBL168 OLEANOLIC_ACID IC50 = 35200.0 nM 4.45
CHEMBL470029 OLEANONIC ACID IC50 = 38800.0 nM 4.41
CHEMBL1797142 IC50 = 41300.0 nM 4.38
CHEMBL7890 L-NAME IC50 = 73180.0 nM 4.14