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Assay Detail

CHEMBL3371282

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 20s proteasome subunit beta-5c in human RPMI8226 cells using BODIPY-NC005 by fluorescent densitometry
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type RPMI-8226
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Structure-based design of β1i or β5i specific inhibitors of human immunoproteasomes.
de Bruin G, Huber EM, Xin BT, van Rooden EJ, Al-Ayed K, Kim KB, Kisselev AF, Driessen C, van der Stelt M, van der Marel GA, Groll M, Overkleeft HS.
J Med Chem (2014) 57 : 6197 -6209
PubMed 25006746 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.72 6.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3237875 1 6.75
CHEMBL3319482 1 5.54
CHEMBL3319581 1 5.54
CHEMBL3319481 1 5.38
CHEMBL3319577 1 5.37
CHEMBL3319483 1 -
CHEMBL3319579 1 -
CHEMBL3319591 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3237875 IC50 = 180.0 nM 6.75
CHEMBL3319482 IC50 = 2900.0 nM 5.54
CHEMBL3319581 IC50 = 2900.0 nM 5.54
CHEMBL3319481 IC50 = 4200.0 nM 5.38
CHEMBL3319577 IC50 = 4300.0 nM 5.37
CHEMBL3319483 IC50 > 100000.0 nM -
CHEMBL3319579 IC50 > 100000.0 nM -
CHEMBL3319591 IC50 > 100000.0 nM -