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Assay Detail

CHEMBL3371890

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of protein phosphatase-1 (unknown origin) using phosphopeptide (Lys-Arg-pThr-Ile-Arg) substrate incubated for 60 mins by colorimetric analysis based serine/threonine assay
4
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis and biological evaluation of norcantharidin derivatives as protein phosphatase-1 inhibitors.
Zhao J, Guan XW, Chen SW, Hui L.
Bioorg Med Chem Lett (2015) 25 : 363 -366
PubMed 25466711 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.97 6.70
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3358348 2 6.70
CHEMBL3358355 1 5.64
CHEMBL8327 DEMETHYL-CANTHARIDIN 1 5.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3358348 IC50 = 200.0 nM 6.70
CHEMBL3358355 IC50 = 2300.0 nM 5.64
CHEMBL8327 DEMETHYL-CANTHARIDIN IC50 = 2600.0 nM 5.58
CHEMBL3358348 Inhibition - % -