Assay Detail
Binding
CHEMBL3372399
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full-length Chk1 in HeLa S3 cells assessed as phosphorylation at Ser345 after 4 hrs
5
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HeLa S3 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase Chk1 (CHEMBL4630) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-Based Drug Design of Novel, Potent, and Selective Azabenzimidazoles (ABI) as ATR Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 7.07 | 7.10 |
| EC50 | 2 | 5.86 | 6.24 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3355474 | — | — | 1 | 7.10 |
| CHEMBL3355475 | — | — | 1 | 7.08 |
| CHEMBL3355476 | — | — | 1 | 7.02 |
| CHEMBL3355473 | — | — | 1 | 6.24 |
| CHEMBL3355472 | — | — | 1 | 5.48 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3355474 | — | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL3355475 | — | IC50 | = | 84.0 | nM | 7.08 |
| CHEMBL3355476 | — | IC50 | = | 96.0 | nM | 7.02 |
| CHEMBL3355473 | — | EC50 | = | 570.0 | nM | 6.24 |
| CHEMBL3355472 | — | EC50 | = | 3300.0 | nM | 5.48 |