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Assay Detail

CHEMBL3372941

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human FFA1 expressed in CHO cells assessed as increase in intracellular Ca2+ concentration by Fluo-4 AM based fluorescence assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Free fatty acid receptor 1 (CHEMBL4422)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of phenoxyacetic acid derivatives as novel free fatty acid receptor 1 agonists.
Wang X, Zhao T, Yang B, Li Z, Cui J, Dai Y, Qiu Q, Qiang H, Huang W, Qian H.
Bioorg Med Chem (2015) 23 : 132 -140
PubMed 25481394 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 6.97 7.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1829174 FASIGLIFAM 3.0 1 7.41
CHEMBL3353742 1 7.21
CHEMBL3353741 1 7.03
CHEMBL3353261 1 6.92
CHEMBL3353738 1 6.81
CHEMBL3353262 1 6.41
CHEMBL3353747 1 -
CHEMBL3353746 1 -
CHEMBL3353745 1 -
CHEMBL3353744 1 -
CHEMBL3353743 1 -
CHEMBL3353740 1 -
CHEMBL3353739 1 -
CHEMBL3353263 1 -
CHEMBL3353748 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1829174 FASIGLIFAM EC50 = 38.6 nM 7.41
CHEMBL3353742 EC50 = 62.3 nM 7.21
CHEMBL3353741 EC50 = 92.6 nM 7.03
CHEMBL3353261 EC50 = 121.1 nM 6.92
CHEMBL3353738 EC50 = 153.3 nM 6.81
CHEMBL3353262 EC50 = 389.3 nM 6.41
CHEMBL3353747 EC50 - -
CHEMBL3353746 EC50 - -
CHEMBL3353745 EC50 - -
CHEMBL3353744 EC50 - -
CHEMBL3353743 EC50 - -
CHEMBL3353740 EC50 - -
CHEMBL3353739 EC50 - -
CHEMBL3353263 EC50 - -
CHEMBL3353748 EC50 - -