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Assay Detail

CHEMBL3373000

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human HT-29 cells after 72 hrs under normoxia conditions by SRB assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Autocuration

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of 6-(nitroimidazole-1H-alkyloxyl)-4-anilinoquinazolines as efficient EGFR inhibitors exerting cytotoxic effects both under normoxia and hypoxia.
Cheng W, Zhu S, Ma X, Qiu N, Peng P, Sheng R, Hu Y.
Eur J Med Chem (2015) 89 : 826 -834
PubMed 25462282 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.19 5.73

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3355884 1 5.73
CHEMBL3355875 1 5.66
CHEMBL3355882 1 5.53
CHEMBL3355885 1 5.51
CHEMBL3355881 1 5.45
CHEMBL939 GEFITINIB 4.0 1 5.44
CHEMBL3355878 1 5.35
CHEMBL3355879 1 5.26
CHEMBL3355889 1 5.09
CHEMBL3355874 1 5.08
CHEMBL3355880 1 5.00
CHEMBL3355877 1 4.89
CHEMBL3355883 1 4.81
CHEMBL3355890 1 4.80
CHEMBL3355876 1 4.75
CHEMBL3355888 1 4.63
CHEMBL3355886 1 -
CHEMBL3355887 1 -
CHEMBL3355873 1 -
CHEMBL50882 TIRAPAZAMINE 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3355884 IC50 = 1870.0 nM 5.73
CHEMBL3355875 IC50 = 2210.0 nM 5.66
CHEMBL3355882 IC50 = 2930.0 nM 5.53
CHEMBL3355885 IC50 = 3090.0 nM 5.51
CHEMBL3355881 IC50 = 3550.0 nM 5.45
CHEMBL939 GEFITINIB IC50 = 3630.0 nM 5.44
CHEMBL3355878 IC50 = 4480.0 nM 5.35
CHEMBL3355879 IC50 = 5550.0 nM 5.26
CHEMBL3355889 IC50 = 8210.0 nM 5.09
CHEMBL3355874 IC50 = 8390.0 nM 5.08
CHEMBL3355880 IC50 = 10080.0 nM 5.00
CHEMBL3355877 IC50 = 12890.0 nM 4.89
CHEMBL3355883 IC50 = 15480.0 nM 4.81
CHEMBL3355890 IC50 = 15720.0 nM 4.80
CHEMBL3355876 IC50 = 17580.0 nM 4.75
CHEMBL3355888 IC50 = 23240.0 nM 4.63
CHEMBL3355886 IC50 > 50000.0 nM -
CHEMBL3355887 IC50 > 50000.0 nM -
CHEMBL3355873 IC50 > 50000.0 nM -
CHEMBL50882 TIRAPAZAMINE IC50 > 50000.0 nM -