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Assay Detail

CHEMBL3373417

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human alpha7 nAChR expressed in GH3 cells by Ca2+/Fluo-4 assay
14
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type GH3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor subunit alpha-7 (CHEMBL2492)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Semisynthetic analogues of toxiferine I and their pharmacological properties at α7 nAChRs, muscle-type nAChRs, and the allosteric binding site of muscarinic M2 receptors.
Zlotos DP, Tränkle C, Holzgrabe U, Gündisch D, Jensen AA.
J Nat Prod (2014) 77 : 2006 -2013
PubMed 25192059 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.57 6.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3337862 2 6.23
CHEMBL3337866 2 6.09
CHEMBL3337861 2 5.66
CHEMBL3337863 2 5.51
CHEMBL3337865 2 5.48
CHEMBL3355097 2 5.38
CHEMBL3355098 2 4.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3337862 IC50 = 590.0 nM 6.23
CHEMBL3337862 IC50 = 602.56 nM 6.22
CHEMBL3337866 IC50 = 812.83 nM 6.09
CHEMBL3337866 IC50 = 820.0 nM 6.09
CHEMBL3337861 IC50 = 2200.0 nM 5.66
CHEMBL3337861 IC50 = 2344.23 nM 5.63
CHEMBL3337863 IC50 = 3100.0 nM 5.51
CHEMBL3337863 IC50 = 3162.28 nM 5.50
CHEMBL3337865 IC50 = 3311.31 nM 5.48
CHEMBL3337865 IC50 = 3300.0 nM 5.48
CHEMBL3355097 IC50 = 4168.69 nM 5.38
CHEMBL3355097 IC50 = 4200.0 nM 5.38
CHEMBL3355098 IC50 = 21000.0 nM 4.68
CHEMBL3355098 IC50 = 21379.62 nM 4.67