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Assay Detail

CHEMBL3374010

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK2 (unknown origin)-mediated phosphorylation of Biotin-KAIETDKEYYTVKD incubated for 10 mins prior to substrate addition measured after 1 hr in presence of [gamma-33P]ATP by scintillation counting analysis
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Scaffold hopping towards potent and selective JAK3 inhibitors: discovery of novel C-5 substituted pyrrolopyrazines.
de Vicente J, Lemoine R, Bartlett M, Hermann JC, Hekmat-Nejad M, Henningsen R, Jin S, Kuglstatter A, Li H, Lovey AJ, Menke J, Niu L, Patel V, Petersen A, Setti L, Shao A, Tivitmahaisoon P, Vu MD, Soth M.
Bioorg Med Chem Lett (2014) 24 : 4969 -4975
PubMed 25262541 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 7.17 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL221959 TOFACITINIB 4.0 1 8.40
CHEMBL3335678 1 8.15
CHEMBL3335691 1 8.05
CHEMBL3335686 1 7.80
CHEMBL3335690 1 7.62
CHEMBL3357978 1 7.44
CHEMBL3335699 1 7.35
CHEMBL3335685 1 7.33
CHEMBL3335676 1 7.33
CHEMBL3335682 1 7.32
CHEMBL3335692 1 7.29
CHEMBL3335687 1 7.25
CHEMBL3335694 1 7.15
CHEMBL3335684 1 7.11
CHEMBL3335688 1 7.09
CHEMBL3335680 1 7.04
CHEMBL3335683 1 7.00
CHEMBL3335698 1 6.99
CHEMBL3335689 1 6.97
CHEMBL3335695 1 6.89
CHEMBL3335697 1 6.75
CHEMBL3335681 1 6.74
CHEMBL3335693 1 6.74
CHEMBL3335677 1 6.65
CHEMBL3335679 1 6.63
CHEMBL3357977 1 5.36
CHEMBL3335696 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL221959 TOFACITINIB IC50 = 4.0 nM 8.40
CHEMBL3335678 IC50 = 7.0 nM 8.15
CHEMBL3335691 IC50 = 9.0 nM 8.05
CHEMBL3335686 IC50 = 16.0 nM 7.80
CHEMBL3335690 IC50 = 24.0 nM 7.62
CHEMBL3357978 IC50 = 36.0 nM 7.44
CHEMBL3335699 IC50 = 45.0 nM 7.35
CHEMBL3335685 IC50 = 47.0 nM 7.33
CHEMBL3335676 IC50 = 47.0 nM 7.33
CHEMBL3335682 IC50 = 48.0 nM 7.32
CHEMBL3335692 IC50 = 51.0 nM 7.29
CHEMBL3335687 IC50 = 56.0 nM 7.25
CHEMBL3335694 IC50 = 71.0 nM 7.15
CHEMBL3335684 IC50 = 78.0 nM 7.11
CHEMBL3335688 IC50 = 82.0 nM 7.09
CHEMBL3335680 IC50 = 92.0 nM 7.04
CHEMBL3335683 IC50 = 101.0 nM 7.00
CHEMBL3335698 IC50 = 102.0 nM 6.99
CHEMBL3335689 IC50 = 108.0 nM 6.97
CHEMBL3335695 IC50 = 129.0 nM 6.89
CHEMBL3335697 IC50 = 180.0 nM 6.75
CHEMBL3335681 IC50 = 181.0 nM 6.74
CHEMBL3335693 IC50 = 181.0 nM 6.74
CHEMBL3335677 IC50 = 226.0 nM 6.65
CHEMBL3335679 IC50 = 234.0 nM 6.63
CHEMBL3357977 IC50 = 4410.0 nM 5.36
CHEMBL3335696 IC50 = 54.0 nM -