Assay Detail
Binding
CHEMBL3375306
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to human CDK4/cyclinD1 using GST-pRB152 as substrate by chemiluminescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Cyclin-dependent kinase 4/cyclin D1 (CHEMBL1907601) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Biphenyl-4-carboxylic acid [2-(1H-indol-3-yl)-ethyl]-methylamide (CA224), a nonplanar analogue of fascaplysin, inhibits Cdk4 and tubulin polymerization: evaluation of in vitro and in vivo anticancer activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.08 | 6.39 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL258765 | FASCAPLYSIN CHLORIDE | — | 1 | 6.39 |
| CHEMBL213805 | — | — | 1 | 5.21 |
| CHEMBL213302 | — | — | 1 | 4.42 |
| CHEMBL209467 | — | — | 1 | 4.31 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL258765 | FASCAPLYSIN CHLORIDE | IC50 | = | 410.0 | nM | 6.39 |
| CHEMBL213805 | — | IC50 | = | 6200.0 | nM | 5.21 |
| CHEMBL213302 | — | IC50 | = | 38000.0 | nM | 4.42 |
| CHEMBL209467 | — | IC50 | = | 49000.0 | nM | 4.31 |