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Assay Detail

CHEMBL3381146

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant CK2a1/CK2b holoenzyme expressed in Escherichia coli BL21(DE3) pre-incubated for 10 mins before substrate addition by capillary electrophoresis based assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Casein kinase II (CHEMBL2095191)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

Converting potent indeno[1,2-b]indole inhibitors of protein kinase CK2 into selective inhibitors of the breast cancer resistance protein ABCG2.
Jabor Gozzi G, Bouaziz Z, Winter E, Daflon-Yunes N, Aichele D, Nacereddine A, Marminon C, Valdameri G, Zeinyeh W, Bollacke A, Guillon J, Lacoudre A, Pinaud N, Cadena SM, Jose J, Le Borgne M, Di Pietro A.
J Med Chem (2015) 58 : 265 -277
PubMed 25272055 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.79 8.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1230165 SILMITASERTIB 2.0 1 8.43
CHEMBL3353420 1 7.60
CHEMBL6246 ELLAGIC ACID 2.0 1 7.40
CHEMBL177820 TETRABROMOBENZOTRIAZOLE 1 7.22
CHEMBL3353413 1 6.77
CHEMBL1957190 1 6.44
CHEMBL289277 EMODIN 1 6.24
CHEMBL3353416 1 5.85
CHEMBL3353414 1 5.60
CHEMBL3353418 1 5.39
CHEMBL3353417 1 5.29
CHEMBL1957193 1 5.22
CHEMBL1957192 1 5.16
CHEMBL3353421 1 5.04
CHEMBL3353422 1 4.96
CHEMBL3353419 1 4.92
CHEMBL1957191 1 4.82
CHEMBL3353415 1 4.82
CHEMBL1957203 1 4.46
CHEMBL1957202 1 4.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1230165 SILMITASERTIB IC50 = 3.7 nM 8.43
CHEMBL3353420 IC50 = 25.0 nM 7.60
CHEMBL6246 ELLAGIC ACID IC50 = 40.0 nM 7.40
CHEMBL177820 TETRABROMOBENZOTRIAZOLE IC50 = 60.0 nM 7.22
CHEMBL3353413 IC50 = 170.0 nM 6.77
CHEMBL1957190 IC50 = 360.0 nM 6.44
CHEMBL289277 EMODIN IC50 = 580.0 nM 6.24
CHEMBL3353416 IC50 = 1400.0 nM 5.85
CHEMBL3353414 IC50 = 2500.0 nM 5.60
CHEMBL3353418 IC50 = 4100.0 nM 5.39
CHEMBL3353417 IC50 = 5100.0 nM 5.29
CHEMBL1957193 IC50 = 6000.0 nM 5.22
CHEMBL1957192 IC50 = 7000.0 nM 5.16
CHEMBL3353421 IC50 = 9200.0 nM 5.04
CHEMBL3353422 IC50 = 11000.0 nM 4.96
CHEMBL3353419 IC50 = 12000.0 nM 4.92
CHEMBL1957191 IC50 = 15000.0 nM 4.82
CHEMBL3353415 IC50 = 15000.0 nM 4.82
CHEMBL1957203 IC50 = 35000.0 nM 4.46
CHEMBL1957202 IC50 = 55000.0 nM 4.26