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Assay Detail

CHEMBL3385677

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Binding
Agonist activity at human alpha4beta2 nAChR expressed in SH-EP1 cells by [86Rb+] ion efflux assay
14
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-EP1
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Enantiopure Cyclopropane-Bearing Pyridyldiazabicyclo[3.3.0]octanes as Selective α4β2-nAChR Ligands.
Onajole OK, Eaton JB, Lukas RJ, Brunner D, Thiede L, Caldarone BJ, Kozikowski AP.
ACS Med Chem Lett (2014) 5 : 1196 -1201
PubMed 25408831 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 7.58 8.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2024096 1 8.24
CHEMBL3356148 2 7.90
CHEMBL3356150 2 7.80
CHEMBL2024094 1 7.75
CHEMBL3356152 2 7.70
CHEMBL3356151 2 7.60
CHEMBL3 NICOTINE 4.0 2 6.52
CHEMBL3356149 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2024096 EC50 = 5.8 nM 8.24
CHEMBL3356148 EC50 = 12.59 nM 7.90
CHEMBL3356148 EC50 = 14.0 nM 7.85
CHEMBL3356150 EC50 = 15.85 nM 7.80
CHEMBL2024094 EC50 = 18.0 nM 7.75
CHEMBL3356150 EC50 = 18.0 nM 7.75
CHEMBL3356152 EC50 = 20.0 nM 7.70
CHEMBL3356152 EC50 = 19.95 nM 7.70
CHEMBL3356151 EC50 = 25.0 nM 7.60
CHEMBL3356151 EC50 = 25.12 nM 7.60
CHEMBL3 NICOTINE EC50 = 300.0 nM 6.52
CHEMBL3 NICOTINE EC50 = 316.23 nM 6.50
CHEMBL3356149 EC50 > 1000.0 nM -
CHEMBL3356149 EC50 > 1000.0 nM -