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Assay Detail

CHEMBL3385954

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human TLR4 expressed in HEK293 cells coexpressed with human MD2/CD14 assessed as activation of NFkappaB signaling after 20 to 24 hrs by NFkappaB reporter assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Toll-like receptor 4 (CHEMBL5255)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of αGlcN(1↔1)αMan-based lipid A mimetics as a novel class of potent Toll-like receptor 4 agonists.
Adanitsch F, Ittig S, Stöckl J, Oblak A, Haegman M, Jerala R, Beyaert R, Kosma P, Zamyatina A.
J Med Chem (2014) 57 : 8056 -8071
PubMed 25252784 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 9.00 10.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3354640 1 10.10
CHEMBL3354639 1 9.40
CHEMBL3354641 1 7.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3354640 EC50 = 0.08 nM 10.10
CHEMBL3354639 EC50 = 0.4 nM 9.40
CHEMBL3354641 EC50 = 31.0 nM 7.51