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Assay Detail

CHEMBL3387656

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse GPR119 overexpressed in CHO cells assessed as increase in cellular cAMP production by HTRF method
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucose-dependent insulinotropic receptor (CHEMBL5263)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Novel tricyclic pyrazolopyrimidines as potent and selective GPR119 agonists.
Azimioara M, Alper P, Cow C, Mutnick D, Nikulin V, Lelais G, Mecom J, McNeill M, Michellys PY, Wang Z, Reding E, Paliotti M, Li J, Bao D, Zoll J, Kim Y, Zimmerman M, Groessl T, Tuntland T, Joseph SB, McNamara P, Seidel HM, Epple R.
Bioorg Med Chem Lett (2014) 24 : 5478 -5483
PubMed 25455488 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 7.75 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3354777 1 8.10
CHEMBL3354778 1 8.10
CHEMBL3354780 1 8.05
CHEMBL3354774 1 7.96
CHEMBL3354784 1 7.96
CHEMBL3354791 1 7.85
CHEMBL3354788 1 7.82
CHEMBL3354779 1 7.77
CHEMBL3354776 1 7.62
CHEMBL3354789 1 7.60
CHEMBL3354781 1 7.57
CHEMBL3354790 1 7.46
CHEMBL3354793 1 6.87

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3354777 EC50 = 8.0 nM 8.10
CHEMBL3354778 EC50 = 8.0 nM 8.10
CHEMBL3354780 EC50 = 9.0 nM 8.05
CHEMBL3354774 EC50 = 11.0 nM 7.96
CHEMBL3354784 EC50 = 11.0 nM 7.96
CHEMBL3354791 EC50 = 14.0 nM 7.85
CHEMBL3354788 EC50 = 15.0 nM 7.82
CHEMBL3354779 EC50 = 17.0 nM 7.77
CHEMBL3354776 EC50 = 24.0 nM 7.62
CHEMBL3354789 EC50 = 25.0 nM 7.60
CHEMBL3354781 EC50 = 27.0 nM 7.57
CHEMBL3354790 EC50 = 35.0 nM 7.46
CHEMBL3354793 EC50 = 135.0 nM 6.87