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Assay Detail

CHEMBL3389006

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human IDH1 R132C mutant expressed in Escherichia coli BL21-CodonPlus assessed as reduction in NADPH consumption
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibition of cancer-associated mutant isocitrate dehydrogenases: synthesis, structure-activity relationship, and selective antitumor activity.
Liu Z, Yao Y, Kogiso M, Zheng B, Deng L, Qiu JJ, Dong S, Lv H, Gallo JM, Li XN, Song Y.
J Med Chem (2014) 57 : 8307 -8318
PubMed 25271760 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 5.95 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2386125 1 6.92
CHEMBL3343444 1 6.58
CHEMBL2386128 1 6.57
CHEMBL3343445 1 6.38
CHEMBL3343463 1 6.25
CHEMBL3343464 1 6.21
CHEMBL3343462 1 6.10
CHEMBL2386127 1 5.75
CHEMBL2386129 1 5.62
CHEMBL3343434 1 5.18
CHEMBL2386126 1 4.98
CHEMBL3343439 1 4.83

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2386125 Ki = 120.0 nM 6.92
CHEMBL3343444 Ki = 260.0 nM 6.58
CHEMBL2386128 Ki = 270.0 nM 6.57
CHEMBL3343445 Ki = 420.0 nM 6.38
CHEMBL3343463 Ki = 560.0 nM 6.25
CHEMBL3343464 Ki = 620.0 nM 6.21
CHEMBL3343462 Ki = 800.0 nM 6.10
CHEMBL2386127 Ki = 1800.0 nM 5.75
CHEMBL2386129 Ki = 2400.0 nM 5.62
CHEMBL3343434 Ki = 6600.0 nM 5.18
CHEMBL2386126 Ki = 10500.0 nM 4.98
CHEMBL3343439 Ki = 14700.0 nM 4.83