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Assay Detail

CHEMBL3390165

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human PPARgamma expressed in HEK293 cells incubated for 18 hrs by luciferase reporter gene assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Polyyne hybrid compounds from Notopterygium incisum with peroxisome proliferator-activated receptor gamma agonistic effects.
Liu X, Kunert O, Blunder M, Fakhrudin N, Noha SM, Malainer C, Schinkovitz A, Heiss EH, Atanasov AG, Kollroser M, Schuster D, Dirsch VM, Bauer R.
J Nat Prod (2014) 77 : 2513 -2521
PubMed 25333853 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 5.88 6.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL595 PIOGLITAZONE 4.0 1 6.68
CHEMBL3358034 1 5.77
CHEMBL3358037 1 5.77
CHEMBL3358033 1 5.72
CHEMBL3358035 1 5.70
CHEMBL3358036 1 5.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL595 PIOGLITAZONE EC50 = 210.0 nM 6.68
CHEMBL3358034 EC50 = 1700.0 nM 5.77
CHEMBL3358037 EC50 = 1700.0 nM 5.77
CHEMBL3358033 EC50 = 1900.0 nM 5.72
CHEMBL3358035 EC50 = 2000.0 nM 5.70
CHEMBL3358036 EC50 = 2300.0 nM 5.64