Assay Detail
Binding
CHEMBL3395144
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant Plk1 (unknown origin) preincubated for 30 mins before recombinant Cdc25C substrate addition by ADP-Glo kinase assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase PLK1 (CHEMBL3024) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Identification of indole-3-carboxylic acids as non-ATP-competitive Polo-like kinase 1 (Plk1) inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 14 | 5.93 | 8.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1241855 | RIGOSERTIB | 3.0 | 1 | 8.05 |
| CHEMBL3394764 | — | — | 1 | 6.89 |
| CHEMBL3394763 | — | — | 1 | 6.39 |
| CHEMBL3394766 | — | — | 1 | 6.02 |
| CHEMBL3394757 | — | — | 1 | 5.74 |
| CHEMBL3394765 | — | — | 1 | 5.73 |
| CHEMBL3394758 | — | — | 1 | 5.72 |
| CHEMBL3394759 | — | — | 1 | 5.68 |
| CHEMBL1672002 | THYMOQUINONE | 2.0 | 1 | 5.66 |
| CHEMBL3394762 | — | — | 1 | 5.58 |
| CHEMBL3394768 | — | — | 1 | 5.54 |
| CHEMBL3394761 | — | — | 1 | 5.46 |
| CHEMBL3394760 | — | — | 1 | 5.32 |
| CHEMBL3394767 | — | — | 1 | 5.24 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1241855 | RIGOSERTIB | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL3394764 | — | IC50 | = | 130.0 | nM | 6.89 |
| CHEMBL3394763 | — | IC50 | = | 410.0 | nM | 6.39 |
| CHEMBL3394766 | — | IC50 | = | 960.0 | nM | 6.02 |
| CHEMBL3394757 | — | IC50 | = | 1820.0 | nM | 5.74 |
| CHEMBL3394765 | — | IC50 | = | 1880.0 | nM | 5.73 |
| CHEMBL3394758 | — | IC50 | = | 1910.0 | nM | 5.72 |
| CHEMBL3394759 | — | IC50 | = | 2100.0 | nM | 5.68 |
| CHEMBL1672002 | THYMOQUINONE | IC50 | = | 2180.0 | nM | 5.66 |
| CHEMBL3394762 | — | IC50 | = | 2640.0 | nM | 5.58 |
| CHEMBL3394768 | — | IC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL3394761 | — | IC50 | = | 3470.0 | nM | 5.46 |
| CHEMBL3394760 | — | IC50 | = | 4840.0 | nM | 5.32 |
| CHEMBL3394767 | — | IC50 | = | 5710.0 | nM | 5.24 |