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Assay Detail

CHEMBL3395250

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-flumazenil from benzodiazepine receptor in Sprague-Dawley rat brain cortical membranes incubated for 35 mins
14
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Novel agonists of benzodiazepine receptors: design, synthesis, binding assay and pharmacological evaluation of 1,2,4-triazolo[1,5-a]pyrimidinone and 3-amino-1,2,4-triazole derivatives.
Faizi M, Dabirian S, Tajali H, Ahmadi F, Zavareh ER, Shahhosseini S, Tabatabai SA.
Bioorg Med Chem (2015) 23 : 480 -487
PubMed 25564376 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 8.75 9.17
Ki 7 8.96 9.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3394789 2 9.38
CHEMBL3394790 2 9.28
CHEMBL3394788 2 9.01
CHEMBL3394792 2 8.86
CHEMBL3394787 2 8.84
CHEMBL12 DIAZEPAM 4.0 2 8.81
CHEMBL3394791 2 8.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3394789 Ki = 0.42 nM 9.38
CHEMBL3394790 Ki = 0.53 nM 9.28
CHEMBL3394789 IC50 = 0.68 nM 9.17
CHEMBL3394790 IC50 = 0.87 nM 9.06
CHEMBL3394788 Ki = 0.97 nM 9.01
CHEMBL3394792 Ki = 1.37 nM 8.86
CHEMBL3394787 Ki = 1.46 nM 8.84
CHEMBL12 DIAZEPAM Ki = 1.53 nM 8.81
CHEMBL3394788 IC50 = 1.59 nM 8.80
CHEMBL3394792 IC50 = 2.24 nM 8.65
CHEMBL3394787 IC50 = 2.39 nM 8.62
CHEMBL12 DIAZEPAM IC50 = 2.5 nM 8.60
CHEMBL3394791 Ki = 2.73 nM 8.56
CHEMBL3394791 IC50 = 4.45 nM 8.35