Assay Detail
Binding
CHEMBL3398877
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity against rat recombinant TRPA1 expressed in HEK293 cells assessed as inhibition of AITC-induced Ca2+ influx pre-treated 5 mins before capsaicin addition by Fluo-4 dye based assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily A member 1 (CHEMBL5160) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Discovery of non-electrophilic capsaicinoid-type TRPA1 ligands.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 5.27 | 6.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3398239 | — | — | 1 | 6.00 |
| CHEMBL3398238 | — | — | 1 | 5.77 |
| CHEMBL3398241 | — | — | 1 | 5.54 |
| CHEMBL3398240 | — | — | 1 | 4.95 |
| CHEMBL233248 | ALLYL ISOTHIOCYANATE | 1.0 | 1 | 4.07 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3398239 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL3398238 | — | IC50 | = | 1710.0 | nM | 5.77 |
| CHEMBL3398241 | — | IC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL3398240 | — | IC50 | = | 11200.0 | nM | 4.95 |
| CHEMBL233248 | ALLYL ISOTHIOCYANATE | IC50 | = | 85900.0 | nM | 4.07 |