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Assay Detail

CHEMBL3398933

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminally FLAG-tagged wild type EZH2 in EZH2/SUZ12/EED/RbAp48 complex (unknown origin) expressed in baculovirus infected in SF9 cells assessed as inhibition of methylation of nucleosomes at H3K27 by scintillation counting in presence of [3H]SAM
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone-lysine N-methyltransferase EZH2 (CHEMBL2189110)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SAH derived potent and selective EZH2 inhibitors.
Kung PP, Huang B, Zehnder L, Tatlock J, Bingham P, Krivacic C, Gajiwala K, Diehl W, Yu X, Maegley KA.
Bioorg Med Chem Lett (2015) 25 : 1532 -1537
PubMed 25746813 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 4.84 6.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3397332 1 6.57
CHEMBL3397325 1 5.85
CHEMBL3397329 1 5.52
CHEMBL3397327 1 5.10
CHEMBL418052 S-ADENOSYLHOMOCYSTEINE 1 4.96
CHEMBL3397330 1 4.85
CHEMBL3397316 1 4.72
CHEMBL3397323 1 4.62
CHEMBL3397315 1 4.62
CHEMBL3397324 1 4.55
CHEMBL3397328 1 4.40
CHEMBL3397331 1 4.40
CHEMBL3397319 1 4.40
CHEMBL3397326 1 4.30
CHEMBL3397320 1 4.30
CHEMBL3397317 1 4.22
CHEMBL3397318 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3397332 IC50 = 270.0 nM 6.57
CHEMBL3397325 IC50 = 1400.0 nM 5.85
CHEMBL3397329 IC50 = 3000.0 nM 5.52
CHEMBL3397327 IC50 = 8000.0 nM 5.10
CHEMBL418052 S-ADENOSYLHOMOCYSTEINE IC50 = 11000.0 nM 4.96
CHEMBL3397330 IC50 = 14000.0 nM 4.85
CHEMBL3397316 IC50 = 19000.0 nM 4.72
CHEMBL3397323 IC50 = 24000.0 nM 4.62
CHEMBL3397315 IC50 = 24000.0 nM 4.62
CHEMBL3397324 IC50 = 28000.0 nM 4.55
CHEMBL3397328 IC50 = 40000.0 nM 4.40
CHEMBL3397331 IC50 = 40000.0 nM 4.40
CHEMBL3397319 IC50 = 40000.0 nM 4.40
CHEMBL3397326 IC50 = 50000.0 nM 4.30
CHEMBL3397320 IC50 = 50000.0 nM 4.30
CHEMBL3397317 IC50 = 60000.0 nM 4.22
CHEMBL3397318 IC50 = 120000.0 nM -