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Assay Detail

CHEMBL3404113

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human CHT Leu530Ala and Val531Ala mutant expressed in HEK293 cells assessed as remaining transporter activity in presence of 100 nM [3H]choline by [3H]choline uptake assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

High affinity choline transporter 1 (CHEMBL4507)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of a series of 4-methoxy-3-(piperidin-4-yl)oxy benzamides as novel inhibitors of the presynaptic choline transporter.
Bollinger SR, Engers DW, Ennis EA, Wright J, Locuson CW, Lindsley CW, Blakely RD, Hopkins CR.
Bioorg Med Chem Lett (2015) 25 : 1757 -1760
PubMed 25801932 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 5.68 6.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1872483 1 6.62
CHEMBL3304438 1 6.57
CHEMBL3303792 1 6.12
CHEMBL3303292 1 5.90
CHEMBL3182315 1 5.80
CHEMBL3183447 1 5.67
CHEMBL3187862 1 5.46
CHEMBL3188567 1 5.38
CHEMBL3187348 1 5.31
CHEMBL3303803 1 5.21
CHEMBL3186365 1 5.09
CHEMBL3183383 1 5.03
CHEMBL3183609 1 -
CHEMBL3401758 1 -
CHEMBL3301712 1 -
CHEMBL3301821 1 -
CHEMBL3187345 1 -
CHEMBL3188740 1 -
CHEMBL3304288 1 -
CHEMBL3184436 1 -
CHEMBL1878664 1 -
CHEMBL3184012 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1872483 IC50 = 240.0 nM 6.62
CHEMBL3304438 IC50 = 270.0 nM 6.57
CHEMBL3303792 IC50 = 760.0 nM 6.12
CHEMBL3303292 IC50 = 1250.0 nM 5.90
CHEMBL3182315 IC50 = 1580.0 nM 5.80
CHEMBL3183447 IC50 = 2130.0 nM 5.67
CHEMBL3187862 IC50 = 3480.0 nM 5.46
CHEMBL3188567 IC50 = 4120.0 nM 5.38
CHEMBL3187348 IC50 = 4930.0 nM 5.31
CHEMBL3303803 IC50 = 6120.0 nM 5.21
CHEMBL3186365 IC50 = 8090.0 nM 5.09
CHEMBL3183383 IC50 = 9270.0 nM 5.03
CHEMBL3183609 IC50 - -
CHEMBL3401758 IC50 - -
CHEMBL3301712 IC50 - -
CHEMBL3301821 IC50 - -
CHEMBL3187345 IC50 - -
CHEMBL3188740 IC50 - -
CHEMBL3304288 IC50 - -
CHEMBL3184436 IC50 - -
CHEMBL1878664 IC50 - -
CHEMBL3184012 IC50 - -