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Assay Detail

CHEMBL3404592

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of calf intestinal alkaline phosphatase using CDP-star chemiluminescent substrate assessed as change in luminescence by spectrophotometric method
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Intestinal-type alkaline phosphatase (CHEMBL5695)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Diarylsulfonamides and their bioisosteres as dual inhibitors of alkaline phosphatase and carbonic anhydrase: Structure activity relationship and molecular modelling studies.
Al-Rashida M, Ejaz SA, Ali S, Shaukat A, Hamayoun M, Ahmed M, Iqbal J.
Bioorg Med Chem (2015) 23 : 2435 -2444
PubMed 25865133 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.44 6.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3403324 1 6.85
CHEMBL3403318 1 6.37
CHEMBL3403328 1 6.14
CHEMBL23922 1 5.95
CHEMBL3403320 1 5.93
CHEMBL3403327 1 5.90
CHEMBL511186 1 5.87
CHEMBL1607835 1 5.53
CHEMBL23559 1 5.52
CHEMBL3403317 1 5.35
CHEMBL3403319 1 5.23
CHEMBL3403321 1 5.17
CHEMBL3403325 1 5.08
CHEMBL182659 1 5.04
CHEMBL3403322 1 5.01
CHEMBL3403326 1 4.93
CHEMBL3403323 1 4.84
CHEMBL3403329 1 4.52
CHEMBL301523 PHENYLALANINE 3.0 1 4.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3403324 IC50 = 140.0 nM 6.85
CHEMBL3403318 IC50 = 429.0 nM 6.37
CHEMBL3403328 IC50 = 720.0 nM 6.14
CHEMBL23922 IC50 = 1130.0 nM 5.95
CHEMBL3403320 IC50 = 1180.0 nM 5.93
CHEMBL3403327 IC50 = 1270.0 nM 5.90
CHEMBL511186 IC50 = 1350.0 nM 5.87
CHEMBL1607835 IC50 = 2960.0 nM 5.53
CHEMBL23559 IC50 = 3050.0 nM 5.52
CHEMBL3403317 IC50 = 4450.0 nM 5.35
CHEMBL3403319 IC50 = 5910.0 nM 5.23
CHEMBL3403321 IC50 = 6730.0 nM 5.17
CHEMBL3403325 IC50 = 8270.0 nM 5.08
CHEMBL182659 IC50 = 9150.0 nM 5.04
CHEMBL3403322 IC50 = 9750.0 nM 5.01
CHEMBL3403326 IC50 = 11700.0 nM 4.93
CHEMBL3403323 IC50 = 14500.0 nM 4.84
CHEMBL3403329 IC50 = 30100.0 nM 4.52
CHEMBL301523 PHENYLALANINE IC50 = 80210.0 nM 4.10