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Assay Detail

CHEMBL3406977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant rat soluble epoxide hydrolase assessed as increase in fluorescence intensity by fluorescence plate reader
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bifunctional epoxide hydrolase 2 (CHEMBL5669)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Three-dimensional rational approach to the discovery of potent substituted cyclopropyl urea soluble epoxide hydrolase inhibitors.
Takai K, Chiyo N, Nakajima T, Nariai T, Ishikawa C, Nakatani S, Ikeno A, Yamamoto S, Sone T.
Bioorg Med Chem Lett (2015) 25 : 1705 -1708
PubMed 25800114 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 8.52 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3401636 1 9.70
CHEMBL3401639 1 9.70
CHEMBL3401638 1 9.10
CHEMBL3401635 1 8.49
CHEMBL3401634 1 8.41
CHEMBL3401632 1 7.93
CHEMBL3401637 1 7.56
CHEMBL3401630 1 7.25
CHEMBL3401631 1 -
CHEMBL3401633 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3401636 IC50 = 0.2 nM 9.70
CHEMBL3401639 IC50 = 0.2 nM 9.70
CHEMBL3401638 IC50 = 0.8 nM 9.10
CHEMBL3401635 IC50 = 3.2 nM 8.49
CHEMBL3401634 IC50 = 3.9 nM 8.41
CHEMBL3401632 IC50 = 11.7 nM 7.93
CHEMBL3401637 IC50 = 27.7 nM 7.56
CHEMBL3401630 IC50 = 55.6 nM 7.25
CHEMBL3401631 IC50 > 100.0 nM -
CHEMBL3401633 IC50 > 100.0 nM -