Assay Detail
Binding
CHEMBL3411013
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of macrophage migration inhibitory factor tautomerase activity in human Jurkat T cells using L-dopachrome methyl ester as substrate incubated for 30 mins prior to substrate addition measured 24 months post determination of initial value by spectrophotometric analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Jurkat T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Multiple binding modes of isothiocyanates that inhibit macrophage migration inhibitory factor.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 4.99 | 5.02 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3409226 | — | — | 1 | 5.02 |
| CHEMBL3409227 | — | — | 1 | 4.96 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3409226 | — | IC50 | = | 9600.0 | nM | 5.02 |
| CHEMBL3409227 | — | IC50 | = | 11000.0 | nM | 4.96 |