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Assay Detail

CHEMBL3412490

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Negative allosteric modulation of mGlu5 (unknown origin) expressed in HEK293 cells assessed as inhibition of L-AP4-induced calcium mobilization incubated for 30 mins prior to L-AP4 induction by Fluo-4 AM staining-based fluorescence assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 5 (CHEMBL3227)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Metabotropic glutamate receptor 5 negative allosteric modulators: discovery of 2-chloro-4-[1-(4-fluorophenyl)-2,5-dimethyl-1H-imidazol-4-ylethynyl]pyridine (basimglurant, RO4917523), a promising novel medicine for psychiatric diseases.
Jaeschke G, Kolczewski S, Spooren W, Vieira E, Bitter-Stoll N, Boissin P, Borroni E, Büttelmann B, Ceccarelli S, Clemann N, David B, Funk C, Guba W, Harrison A, Hartung T, Honer M, Huwyler J, Kuratli M, Niederhauser U, Pähler A, Peters JU, Petersen A, Prinssen E, Ricci A, Rueher D, Rueher M, Schneider M, Spurr P, Stoll T, Tännler D, Wichmann J, Porter RH, Wettstein JG, Lindemann L.
J Med Chem (2015) 58 : 1358 -1371
PubMed 25565255 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 7.11 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3301626 BASIMGLURANT 2.0 1 8.15
CHEMBL3410213 1 8.05
CHEMBL3410223 1 8.00
CHEMBL3410214 1 7.96
CHEMBL3410220 1 7.92
CHEMBL3410215 1 7.89
CHEMBL3410224 1 7.80
CHEMBL3410209 1 7.58
CHEMBL3410216 1 7.55
CHEMBL1946770 1 7.55
CHEMBL3410217 1 7.16
CHEMBL3410221 1 7.01
CHEMBL3410218 1 7.00
CHEMBL3410211 1 6.91
CHEMBL3410219 1 6.85
CHEMBL3410207 1 6.79
CHEMBL3407528 1 6.53
CHEMBL3410212 1 6.41
CHEMBL3410225 1 6.31
CHEMBL3410222 1 6.06
CHEMBL3410208 1 5.60
CHEMBL3410210 1 5.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3301626 BASIMGLURANT IC50 = 7.0 nM 8.15
CHEMBL3410213 IC50 = 9.0 nM 8.05
CHEMBL3410223 IC50 = 10.0 nM 8.00
CHEMBL3410214 IC50 = 11.0 nM 7.96
CHEMBL3410220 IC50 = 12.0 nM 7.92
CHEMBL3410215 IC50 = 13.0 nM 7.89
CHEMBL3410224 IC50 = 16.0 nM 7.80
CHEMBL3410209 IC50 = 26.0 nM 7.58
CHEMBL3410216 IC50 = 28.0 nM 7.55
CHEMBL1946770 IC50 = 28.0 nM 7.55
CHEMBL3410217 IC50 = 70.0 nM 7.16
CHEMBL3410221 IC50 = 97.0 nM 7.01
CHEMBL3410218 IC50 = 100.0 nM 7.00
CHEMBL3410211 IC50 = 124.0 nM 6.91
CHEMBL3410219 IC50 = 141.0 nM 6.85
CHEMBL3410207 IC50 = 161.0 nM 6.79
CHEMBL3407528 IC50 = 295.0 nM 6.53
CHEMBL3410212 IC50 = 387.0 nM 6.41
CHEMBL3410225 IC50 = 488.0 nM 6.31
CHEMBL3410222 IC50 = 872.0 nM 6.06
CHEMBL3410208 IC50 = 2500.0 nM 5.60
CHEMBL3410210 IC50 = 5600.0 nM 5.25