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Assay Detail

CHEMBL3412922

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of calmodulin-dependent bovine neuronal NOS using [3H]-arginine as substrate assessed as citrulline formation after 30 mins
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Novel 2,4-disubstituted pyrimidines as potent, selective, and cell-permeable inhibitors of neuronal nitric oxide synthase.
Mukherjee P, Li H, Sevrioukova I, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB.
J Med Chem (2015) 58 : 1067 -1088
PubMed 25489882 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 4.60 4.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL275066 1 4.60
CHEMBL540 IMIDAZOLE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL275066 IC50 = 25000.0 nM 4.60
CHEMBL540 IMIDAZOLE IC50 = 200000.0 nM -