Assay Detail
Binding
CHEMBL3418202
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant LSD1 expressed in Escherichia coli using methylated H3K4peptide as substrate incubated for 15 mins prior to substrate addition measured for 12 mins by amplex red staining-based fluorescence assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Lysine-specific histone demethylase 1A (CHEMBL6136) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Pure enantiomers of benzoylamino-tranylcypromine: LSD1 inhibition, gene modulation in human leukemia cells and effects on clonogenic potential of murine promyelocytic blasts.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 6.30 | 7.89 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3416426 | — | — | 1 | 7.89 |
| CHEMBL3416425 | — | — | 1 | 7.72 |
| CHEMBL3416427 | — | — | 1 | 7.68 |
| CHEMBL3416432 | — | — | 1 | 7.66 |
| CHEMBL3416431 | — | — | 1 | 7.58 |
| CHEMBL3416434 | — | — | 1 | 6.35 |
| CHEMBL3416433 | — | — | 1 | 5.56 |
| CHEMBL3416429 | — | — | 1 | 4.96 |
| CHEMBL3416428 | — | — | 1 | 4.93 |
| CHEMBL3989843 | TRANYLCYPROMINE | 4.0 | 1 | 4.87 |
| CHEMBL3416430 | — | — | 1 | 4.13 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3416426 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3416425 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL3416427 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3416432 | — | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL3416431 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL3416434 | — | IC50 | = | 450.0 | nM | 6.35 |
| CHEMBL3416433 | — | IC50 | = | 2730.0 | nM | 5.56 |
| CHEMBL3416429 | — | IC50 | = | 10890.0 | nM | 4.96 |
| CHEMBL3416428 | — | IC50 | = | 11690.0 | nM | 4.93 |
| CHEMBL3989843 | TRANYLCYPROMINE | IC50 | = | 13400.0 | nM | 4.87 |
| CHEMBL3416430 | — | IC50 | = | 74650.0 | nM | 4.13 |