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Assay Detail

CHEMBL3419558

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Binding
Inhibition of recombinant histone acetyltransferase p300 (unknown origin) using dH3-H4 tetramer and [3H]-acetyl-CoA assessed as acetate incorporation after 30 mins by liquid scintillation counting
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone acetyltransferase p300 (CHEMBL3784)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

PAINS in the assay: chemical mechanisms of assay interference and promiscuous enzymatic inhibition observed during a sulfhydryl-scavenging HTS.
Dahlin JL, Nissink JW, Strasser JM, Francis S, Higgins L, Zhou H, Zhang Z, Walters MA.
J Med Chem (2015) 58 : 2091 -2113
PubMed 25634295 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 5.70 6.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3416552 1 6.58
CHEMBL1331211 1 6.48
CHEMBL2030281 1 6.29
CHEMBL2031566 1 6.18
CHEMBL3416536 1 6.00
CHEMBL3416321 1 5.96
CHEMBL3235887 1 5.96
CHEMBL502489 GARCINOL 1 5.77
CHEMBL1385343 1 5.75
CHEMBL3416537 1 5.75
CHEMBL3416335 1 5.72
CHEMBL1600114 1 5.70
CHEMBL3416317 1 5.66
CHEMBL3416526 1 5.55
CHEMBL3235890 1 5.51
CHEMBL3416525 1 5.43
CHEMBL3416316 1 5.21
CHEMBL3416315 1 5.21
CHEMBL3416307 1 5.12
CHEMBL3416528 1 5.01
CHEMBL1602759 1 4.96
CHEMBL106 FLUCONAZOLE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3416552 IC50 = 260.0 nM 6.58
CHEMBL1331211 IC50 = 330.0 nM 6.48
CHEMBL2030281 IC50 = 510.0 nM 6.29
CHEMBL2031566 IC50 = 660.0 nM 6.18
CHEMBL3416536 IC50 = 1000.0 nM 6.00
CHEMBL3416321 IC50 = 1100.0 nM 5.96
CHEMBL3235887 IC50 = 1100.0 nM 5.96
CHEMBL502489 GARCINOL IC50 = 1700.0 nM 5.77
CHEMBL1385343 IC50 = 1800.0 nM 5.75
CHEMBL3416537 IC50 = 1800.0 nM 5.75
CHEMBL3416335 IC50 = 1900.0 nM 5.72
CHEMBL1600114 IC50 = 2000.0 nM 5.70
CHEMBL3416317 IC50 = 2200.0 nM 5.66
CHEMBL3416526 IC50 = 2800.0 nM 5.55
CHEMBL3235890 IC50 = 3100.0 nM 5.51
CHEMBL3416525 IC50 = 3700.0 nM 5.43
CHEMBL3416316 IC50 = 6200.0 nM 5.21
CHEMBL3416315 IC50 = 6200.0 nM 5.21
CHEMBL3416307 IC50 = 7500.0 nM 5.12
CHEMBL3416528 IC50 = 9800.0 nM 5.01
CHEMBL1602759 IC50 = 11000.0 nM 4.96
CHEMBL106 FLUCONAZOLE IC50 - -