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Assay Detail

CHEMBL3420848

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FLAP in calcimycin-stimulated mouse whole blood assessed as inhibition of LTB4 synthesis preincubated for 15 mins followed by calcimycin stimulation measured after 30 mins
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Arachidonate 5-lipoxygenase-activating protein (CHEMBL3414408)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis, SAR, and series evolution of novel oxadiazole-containing 5-lipoxygenase activating protein inhibitors: discovery of 2-[4-(3-{(r)-1-[4-(2-amino-pyrimidin-5-yl)-phenyl]-1-cyclopropyl-ethyl}-[1,2,4]oxadiazol-5-yl)-pyrazol-1-yl]-N,N-dimethyl-acetamide (BI 665915).
Takahashi H, Riether D, Bartolozzi A, Bosanac T, Berger V, Binetti R, Broadwater J, Chen Z, Crux R, De Lombaert S, Dave R, Dines JA, Fadra-Khan T, Flegg A, Garrigou M, Hao MH, Huber J, Hutzler JM, Kerr S, Kotey A, Liu W, Lo HY, Loke PL, Mahaney PE, Morwick TM, Napier S, Olague A, Pack E, Padyana AK, Thomson DS, Tye H, Wu L, Zindell RM, Abeywardane A, Simpson T.
J Med Chem (2015) 58 : 1669 -1690
PubMed 25671290 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.57 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3417531 1 5.82
CHEMBL3417525 BI 665915 1 5.32
CHEMBL3417530 1 -
CHEMBL3417511 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3417531 IC50 = 1500.0 nM 5.82
CHEMBL3417525 BI 665915 IC50 = 4800.0 nM 5.32
CHEMBL3417530 IC50 - -
CHEMBL3417511 IC50 - -