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Assay Detail

CHEMBL3430271

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCB1 in human A2780adr cells pre-incubated for 30 mins followed by calcein AM addition and further incubated for 60 mins by calcein AM assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A2780 ADR
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

HM30181 Derivatives as Novel Potent and Selective Inhibitors of the Breast Cancer Resistance Protein (BCRP/ABCG2).
Köhler SC, Wiese M.
J Med Chem (2015) 58 : 3910 -3921
PubMed 25855895 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.00 6.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL160 CYCLOSPORINE 4.0 1 6.04
CHEMBL3426400 1 5.11
CHEMBL3426393 1 4.96
CHEMBL3426397 1 4.48
CHEMBL3426398 1 4.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL160 CYCLOSPORINE IC50 = 919.0 nM 6.04
CHEMBL3426400 IC50 = 7730.0 nM 5.11
CHEMBL3426393 IC50 = 11000.0 nM 4.96
CHEMBL3426397 IC50 = 32800.0 nM 4.48
CHEMBL3426398 IC50 = 37200.0 nM 4.43